【LWnt-3A細(xì)胞】小鼠皮下結(jié)締組織細(xì)胞
細(xì)胞名稱：小鼠皮下結(jié)締組織細(xì)胞；LWnt-3A
組織來(lái)源：皮下結(jié)締組織細(xì)胞
培養(yǎng)條件：【LWnt-3A細(xì)胞】小鼠皮下結(jié)締組織細(xì)胞DMEM-H:Dulbecco'sModifiedEagle'sMedium(DMEH-214.5g/LiterGlucose)胎牛血清，10%；0.4mg/mlG-418
形態(tài)：貼壁；成纖維細(xì)胞
細(xì)胞來(lái)源：：L-M(TK-)cells(ATCCCCL-1.3)用Wnt-3A表達(dá)載體轉(zhuǎn)染并在含G418的培養(yǎng)基中篩選穩(wěn)轉(zhuǎn)株。Wnt-3A基因編碼一個(gè)有變異的信號(hào)功效分泌性糖蛋白。Wnt基因控制胚胎發(fā)過(guò)程中的許多模式形成和生長(zhǎng)事件。這些細(xì)胞分泌有生物活性的Wnt-3A蛋白。它們是目前生產(chǎn)Wnt-3A條件培養(yǎng)基的來(lái)源。由于這種條件培養(yǎng)基還包含Wnt-3A蛋白外的其他因子，對(duì)于涉及Wnt-3A條件培養(yǎng)基的實(shí)驗(yàn)有必要用其來(lái)源細(xì)胞株(ATCCCRL-2648)作對(duì)照。
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